Completed project · 2022
Metabolic Synergy in Clostridium Co-Cultures for Butanol & Hydrogen Production
Ph.D. Dissertation Research — Distinguished University Fellowship & ENGIE-Axium Fellowship, OSU
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Project overview
Active doctoral project investigating syntrophic synergy between Clostridium carboxidivorans and C. beijerinckii during butanol and hydrogen production from agro-industrial wastes.
Key published outcomes (2026):
• Metabolic synergy of species growth, solvent, and gas production — Bioresource Technology Reports (102802)
• Dynamics of solvent and hydrogen in C. beijerinckii / C. pasteurianum co-cultures — Environmental Technology & Innovation (104755)
The project employs genetic engineering of coenzyme A transferase and butyryl CoA dehydrogenase, co-culture optimization, GC-FID solvent analysis, and HPLC monitoring. Findings were first presented at ASM Microbe 2024 and CFAES Spring 2025 Capstone Poster Presentations.
Research collaborators
Dr. Thaddeus C. Ezeji
Dr. Chima C. Okonkwo
Dr. Samson A. Akinola
Dr. Hasan K. Atiyeh — The Ohio State University
Timeline
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Start
01 Aug 2022
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End
31 May 2026
Completed
Project info
Grant / funder
Ph.D. Dissertation Research — Distinguished University Fellowship & ENGIE-Axium Fellowship, OSU
Institution
The Ohio State University